| Autor: |
Tikumphorn, Sathirapatya, Wikanda, Worrapitirungsi, Poonyapat, Sukawutthiya, Kawin, Rasmeepaisarn, Kornkiat, Vongpaisarnsin |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
International journal of legal medicine. 134(5) |
| ISSN: |
1437-1596 |
| Popis: |
The monitoring of chimerism status in a hematopoietic stem cell transplantation patient is a crucial process and is performed periodically in a short time interval. A short tandem repeat marker is widely used for chimerism analysis due to its high discrimination power. However, the sensitivity of this approach was limited to 5% of a minor contributor and the interpretation is usually interrupted with PCR stochastic phenomena. Here, we developed an SNP panel for chimerism analysis using TaqMan technology. A set of SNPs was selected from Thai ancestry informative markers and open-access databases with proper criteria. We examined the 30 recipient-donor pairs that underwent HSCT and showed that the panel can provide an informative marker from 90% of all pairs. An early detection of artificial chimerism in post-HSCT samples was observed when compared with STR analysis. In addition, the detail of cases was discussed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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