Structural analysis of the 17q22-23 amplicon identifies several independent targets of amplification in breast cancer cell lines and tumors

Autor:	G, Wu, C, Sinclair, S, Hinson, J N, Ingle, P C, Roche, F J, Couch
Rok vydání:	2001
Předmět:	Gene Expression Regulation Neoplastic Reverse Transcriptase Polymerase Chain Reaction Carcinoma Ductal Breast Molecular Sequence Data Gene Amplification Tumor Cells Cultured Humans Breast Neoplasms Amino Acid Sequence Oncogenes Physical Chromosome Mapping Chromosomes Human Pair 17
Zdroj:	Cancer research. 61(13)
ISSN:	0008-5472
Popis:	A novel region of amplification in breast tumors has recently been identified on chromosome 17q22-23. In an effort to identify the oncogenes in the region that are targeted by the amplification process, we determined the structure of the amplicon in breast cancer cell lines and tumors. Physical and transcription maps of the approximately 3.5-Mb region were established and used as the basis for copy number analysis within the region by Southern blot and fluorescence in situ hybridization. Seven specific and independent amplification maxima were identified in breast cancer cell lines and breast tumors. We present correlative amplification and overexpression studies for the FLJ21316 and Hs.6649 genes suggesting a role for these candidates as amplification-dependent oncogenes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::65a8dbd6dc9d564347449d85c37d45b3 https://pubmed.ncbi.nlm.nih.gov/11431322 Zobrazit plný text záznamu