| Autor: |
J F, Coy, I, Velhagen, R, Himmele, H, Delius, A, Poustka, H, Zentgraf |
| Rok vydání: |
1995 |
| Zdroj: |
Cell death and differentiation. 3(2) |
| ISSN: |
1350-9047 |
| Popis: |
A systematic search for genes differentially expressed in human tissues resulted in the isolation of a gene encoding a protein with high homology to DNase I. In addition to the recently described cDNA sequence (Parrish et al., 1995) we have isolated a transcript, alternatively spliced in the 5' noncoding region. The gene is located between the QM and the XAP-2 gene in Xq28 and encodes a 302 amino acid protein with 39% identity to human DNase I. Besides a high homology at the nucleotide and amino acid level, most exon-intron boundaries of DNase I and DNase X are identical, indicating that both genes may have evolved from a common ancestor. The predicted function was verified by expression of a recombinant protein in an inducible bacterial system and detection of DNase activity. In contrast to DNase I a 18 kdal amino terminal fragment of the full length 35 kdal protein exhibited DNase activity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
