δ-Catenin Induced Dendritic Morphogenesis: An Essential Role of p190RhoGEF Interaction through Akt1 Mediated Phosphorylation
| Autor: | Kim, Hangun, Han, Jeong Ran, Park, Jaejun, Oh, Minsoo, James, Sarah E., Chang, Sunghoe, Lu, Qun, Lee, Kwang Youl, Ki, Hyunkyoung, Song, Woo-Joo, Kim, Kwonseop |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Neurons
Threonine Delta Catenin Binding Sites ras-GRF1 Catenins 3T3 Cells Dendritic Cells Embryo Mammalian Phosphoproteins Transfection Hippocampus Article Cell Line GTP Phosphohydrolases Rats Rats Sprague-Dawley Mice Morphogenesis Animals Phosphorylation Cell Adhesion Molecules Proto-Oncogene Proteins c-akt |
| Popis: | Delta-catenin was first identified through its interaction with Presenilin-1 and has been implicated in the regulation of dendrogenesis and cognitive function. However, the molecular mechanisms by which delta-catenin promotes dendritic morphogenesis were unclear. In this study, we demonstrated delta-catenin interaction with p190RhoGEF, and the importance of Akt1-mediated phosphorylation at Thr-454 residue of delta-catenin in this interaction. We have also found that delta-catenin overexpression decreased the binding between p190RhoGEF and RhoA, and significantly lowered the levels of GTP-RhoA but not those of GTP-Rac1 and -Cdc42. Delta-catenin T454A, a defective form in p190RhoGEF binding, did not decrease the binding between p190RhoGEF and RhoA. Delta-catenin T454A also did not lower GTP-RhoA levels and failed to induce dendrite-like process formation in NIH 3T3 fibroblasts. Furthermore, delta-catenin T454A significantly reduced the length and number of mature mushroom shaped spines in primary hippocampal neurons. These results highlight signaling events in the regulation of delta-catenin-induced dendrogenesis and spine morphogenesis. |
| Databáze: | OpenAIRE |
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