| Autor: |
Chen, Andrew W., Saab, George, Jeremic, Aleksandar, Zderic, Vesna |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Ultrasound Med Biol |
| Popis: |
To the best of our knowledge, therapeutic ultrasound (TUS) is thus far an unexplored means of delivering mechanical stimulation to cardiomyocyte cultures which is necessary to engineer a more mature cardiomyocyte phenotype in vitro. Spectral ultrasound (SUS) may provide a way to non-invasively, non-disruptively and inexpensively monitor growth and change in cell cultures over long periods of time. Compared to other measurement methods, SUS as an acoustic measurement tool will not be affected by an acoustic therapy, unlike electrical measurement methods where motion caused by acoustic therapy can impact our measurements. Further SUS has the potential to provide functional as well as morphological information in cell cultures. Human induced pluripotent stem cell cardiomyocytes (iPS-CMs) were imaged with calcium fluorescence microscopy while TUS was being applied. TUS was applied at 600 kHz and 1, 3.4 and 6 W/cm(2) for a continuous one second pulse. Measures of the instantaneous beat frequency, repolarization rate and calcium spike amplitude were calculated from the fluorescence data. 600 kHz TUS at 600 kHz 1 W/cm(2) and 6 W/cm(2) had significant effects in the shortening of both the repolarization rate as well as the instantaneous beat rate of the iPS-CMs (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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