Bioregulation of kallikrein-related peptidases 6, 10 and 11 by the kinin B₁ receptor in breast cancer cells
| Autor: | Pamela, Ehrenfeld, Lorella, Manso, María F, Pavicic, Carola E, Matus, Carlos, Borquez, Alejandro, Lizama, José, Sarmiento, María T, Poblete, Kanti D, Bhoola, Anupan, Naran, Carlos D, Figueroa |
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| Rok vydání: | 2014 |
| Předmět: |
Kininogens
Serine Endopeptidases Down-Regulation Breast Neoplasms Kallidin Bradykinin Receptor Bradykinin B1 Up-Regulation Matrix Metalloproteinase 9 Cell Line Tumor MCF-7 Cells Humans Matrix Metalloproteinase 2 Female Kallikreins Neoplasm Invasiveness RNA Interference RNA Small Interfering Tissue Kallikreins Cell Proliferation |
| Zdroj: | Anticancer research. 34(12) |
| ISSN: | 1791-7530 |
| Popis: | The sera of patients with breast cancer have higher levels of des[Arg(9)]bradykinin, a kinin B1 receptor (B1R) agonist, than that from healthy individuals. Stimulation of breast cancer cells with the analog Lys-des[Arg(9)]bradykinin causes release of metalloproteinases-2 and -9 and increases cell proliferation. We examined the possibility that breast cancer cells, in addition to B1R, express the kinin-forming protease true tissue kallikrein (KLK1) and the endogenous proteins termed kininogens from which kinins are enzymatically released. Furthermore, we investigated whether stimulation of breast cancer cells with a B1R agonist would modify the cellular levels of KLK6, KLK10 and KLK11, three kallikrein-related peptidases with a still poorly-understood biological role in breast cancer. We found that breast cancer cells expressed KLK1 and kininogens, and that stimulation of estrogen-sensitive breast cancer cells with the B1R agonist produced down-regulation of KLK10 (a protease associated with growth suppression) but up-regulation of KLK11 and KLK6 (peptidases related to increased cell proliferation and invasiveness, respectively). Furthermore, we showed that the B1R agonist acts as a functional stimulus for the secretion of KLK1 and KLK6, an event relevant for kinin production and cell invasion, respectively. |
| Databáze: | OpenAIRE |
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