| Autor: |
S N Aisyiyah, Jenie, Yuni, Kusumastuti, Fransiska S H, Krismastuti, Yovilianda M, Untoro, Rizna T, Dewi, Linar Z, Udin, Nina, Artanti |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Sensors (Basel, Switzerland) |
| ISSN: |
1424-8220 |
| Popis: |
The development of fluorescent silica nanoparticles (SNP-RB) from natural amorphous silica and its performance as an Escherichia coli (E. coli) biosensor is described in this paper. SNP-RB was derived from silica recovered from geothermal installation precipitation and modified with the dye, Rhodamine B. The Fourier Infrared (FTIR) confirms the incorporation of Rhodamine B in the silica matrix. Transmission Electron Microscopy (TEM) micrographs show that the SNP-RB had an irregular structure with a particle diameter of about 20–30 nm. The maximum fluorescence spectrum of SNP-RB was recorded at 580 nm, which was further applied to observe the detection performance of the fluorescent nanoparticles towards E. coli. The sensing principle was based on the fluorescence-quenching mechanism of SNP-RB and this provided a wide linear E. coli concentration range of 10–105 CFU/mL with a limit detection of 8 CFU/mL. A rapid response time was observed after only 15 min of incubation of SNP-RB with E. coli. The selectivity of the biosensor was demonstrated and showed that the SNP-RB only gave quenching response only to live E. coli bacteria. The use of SNP-RB as a sensing platform reduced the response time significantly compared to conventional 3-day bacterial assays, as well having excellent analytical performance in terms of sensitivity and selectivity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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