The role of CB
| Autor: | Mustafa A, Karwad, Daniel G, Couch, Elena, Theophilidou, Sarir, Sarmad, David A, Barrett, Michael, Larvin, Karen L, Wright, Jonathan N, Lund, Saoirse E, O'Sullivan |
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| Rok vydání: | 2016 |
| Předmět: |
Inflammation
Polyunsaturated Alkamides Arachidonic Acids Monoacylglycerol Lipases Permeability Amidohydrolases Glycerides Intestines Tissue Culture Techniques Oxygen Consumption Gene Expression Regulation Piperidines Receptor Cannabinoid CB1 Benzamides Electric Impedance Cytokines Humans Benzodioxoles Carbamates Caco-2 Cells Intestinal Mucosa Colorectal Neoplasms Endocannabinoids |
| Zdroj: | FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 31(8) |
| ISSN: | 1530-6860 |
| Popis: | The endocannabinoid system has previously been shown to play a role in the permeability and inflammatory response of the human gut. The goal of our study was to determine the effects of endogenous anandamide (AEA) and 2-arachidonoyl glycerol (2-AG) on the permeability and inflammatory response of intestinal epithelium under normal, inflammatory, and hypoxic conditions. Human intestinal mucosa was modeled using Caco-2 cells. Human tissue was collected from planned colorectal resections. Accumulation of AEA and 2-AG was achieved by inhibiting their metabolizing enzymes URB597 (a fatty acid amide hydrolase inhibitor) and JZL184 (a monoacylglycerol lipase inhibitor). Inflammation and ischemia were simulated with TNF-α and IFN-γ and oxygen deprivation. Permeability changes were measured by transepithelial electrical resistance. The role of the CB |
| Databáze: | OpenAIRE |
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