| Autor: |
Perriotte-Olson, Curtis, Adi, Nikhil, Manickam, Devika S., Westwood, Rachel A., Desouza, Cyrus V., Natarajan, Gopalakrishnan, Crook, Alexandra, Kabanov, Alexander V., Viswanathan, Saraswathi |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
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| Popis: |
OBJECTIVE. An intimate association exists between oxidative stress and inflammation. Because adipose tissue (AT) inflammation is intricately linked to metabolic disorders, we hypothesized that reducing oxidative stress will be effective in ameliorating AT inflammation in obesity. METHODS. Wild type mice were fed a high fat diet (HF) for 8 wk followed by 2 wk treatment with nanoformulated copper/zinc superoxide dismutase (NanoSOD). The mice were divided into: 1) Chow diet, 2) HF, and 3) HF+NanoSOD. RESULTS. The HF+NanoSOD-treated mice showed a significant decrease in plasma and liver triglycerides (TGs) compared to HF-fed mice. Interestingly, NanoSOD reduced the expression of macrophage and inflammatory markers in visceral AT (VAT) and stromal cells derived from VAT. Moreover, the activation of pro-inflammatory signaling pathways, in particular, the extracellular signal-regulated kinases, was blunted in VAT upon NanoSOD treatment. However, markers of oxidative stress were not altered significantly in HF+NanoSOD group in our experimental conditions. Pre-treatment of either macrophages or adipocytes significantly reduced the inflammatory response invoked in an in vitro co-culture system further supporting the role of NanoSOD in inhibiting obesity-linked inflammation. CONCLUSION. Our data suggest that NanoSOD is effective in reducing AT macrophage accumulation and AT inflammation, and also in promoting TG metabolism in obesity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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