First in vitro directed biosynthesis of new compounds by a minimal type II polyketide synthase: evidence for the mechanism of chain length determination

Autor: T P, Nicholson, C, Winfield, J, Westcott, J, Crosby, T J, Simpson, R J, Cox
Rok vydání: 2003
Předmět:
Zdroj: Chemical communications (Cambridge, England). (6)
ISSN: 1359-7345
Popis: The minimal actinorhodin polyketide synthase bearing two point mutations (KSbeta Q161A, ACP C17S) was chemically modified to carry novel C4 to C8 starter units on the ACP: on incubation with an excess of malonyl CoA new 16-carbon polyketides are made, supporting a measuring mechanism.
Databáze: OpenAIRE