Intraepithelial TcR alpha/beta+ lymphocytes express CD45RO more often than the TcR gamma/delta+ counterparts in coeliac disease
| Autor: | Halstensen, T S, Farstad, I N, Scott, H, Fausa, O, Brandtzaeg, P |
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| Jazyk: | angličtina |
| Rok vydání: | 1990 |
| Předmět: |
Adult
Adolescent T-Lymphocytes Receptors Antigen T-Cell Fluorescent Antibody Technique Infant hemic and immune systems chemical and pharmacologic phenomena Middle Aged Antigens Differentiation Epithelium Celiac Disease Jejunum immune system diseases T-Lymphocyte Subsets Child Preschool Histocompatibility Antigens Humans Leukocyte Common Antigens Intestinal Mucosa Child Research Article Aged |
| Popis: | Expression of CD45RO on intraepithelial lymphocytes (IEL) bearing the T-cell receptor (TcR) alpha/beta or gamma/delta was studied in situ by three-colour immunofluorescence on jejunal tissue sections from 21 patients with coeliac disease and eight controls. CD45RA-TcR alpha/beta+ IEL expressed CD45RO significantly more often (75%) than the preferentially expanded TcR gamma/delta+ counterpart (59%). Triple staining for CD3, CD4/8 and CD45RA or CD45RB revealed that all CD3 + 4 - 8 - IEL (taken to be TcR gamma/delta+) expressed CD45RB and none were CD45RA. CD45RO positivity was of the same magnitude (66%) on the predominating monoclonal antibody delta TCS1-reactive fraction of TcR gamma/delta+ cells as on the remainder of the TcR gamma/delta+ subset. These results suggest that gluten exposition in patients with coeliac disease leads to accumulation of CD45RA-, putative antigen-primed memory cells of both TcR phenotypes. The less marked CD45RO expression within the preferentially expanded TcR gamma/delta+ subset of IEL may be of particular biological interest. |
| Databáze: | OpenAIRE |
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