[Physico-chemical properties of swine vesicular disease virus]

Autor: H, Liebermann, P, Schulze, R, Riebe, R, Koitzsch
Jazyk: němčina
Rok vydání: 1976
Předmět:
Zdroj: Archiv fur experimentelle Veterinarmedizin. 30(3)
ISSN: 0003-9055
Popis: Titration of SVDV on primary pig kidney cell cultures revealed a plating efficiency of less than or equal to 0,9 X 10(-3). Concentration and purification of the SVD-Virus propagated on pig kidney cell cultures were done by chloroform treatment, adsorption, differential- and density gradient centrifugation. The following physical parameters were found: SVDV is an isometrical RNA-virus having a diameter of 25,1 +/- 1,0 nm. It is resistent to the action of chloroform, ether and pH. The virus has a sedimentation coefficient of 156 +/- 3S and a bouyant density in CsCl of 1,33 +/- 0,01 g/ml. Within the family of picornaviruses the SVDV belongs to the subgroup of enteroviruses and can be distinguished from the foot-and-mouth disease virus by the difference in pH-sensitivity and bouyant density in CsCl.
Databáze: OpenAIRE