| Autor: |
Toi, Van Pham, Phuong, Pham Nguyen, Tho, Nguyen Duc Khanh, Nhien, Nguyen Thanh Thuy, Ca, Nguyen Thuy Nha, Thomas, Pouplin, Jeremy, Farrar, Guy E, Thwaites, Hien, Tran Tinh |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Biomedical Chromatography |
| ISSN: |
1099-0801 |
| Popis: |
A sensitive, simple method for quantification of chloroquine (CQ) and desethylchloroquine (MCQ) in whole blood and plasma from Plasmodium vivax patients has been developed using HPLC with diode array detection (DAD). Solid‐phase extraction on Isolute‐96‐CBA was employed to process 100 μL of plasma/whole blood samples. CQ, MCQ and quinine were separated using a mobile phase of phosphate buffer 25 mm, pH 2.60–acetonitrile (88:12, v/v) with 2 mm sodium perchlorate on a Zorbax SB‐CN 150 × 4.6 mm, 5 μm column at a flow rate of 1.2 mL/min, at ambient temperature in 10 min, with the DAD wavelength of 343 nm. The method was linear over the range of 10–5000 ng/mL for both CQ and MCQ in plasma and whole blood. The limit of detection was 4 ng/mL and limit of quantification was 10 ng/mL in both plasma and blood for CQ and MCQ. The intra‐, inter‐ and total assay precision were |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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