Development of EST-SSR markers of Ipomoea nil

Autor:	Ly, Tong, Fukuoka, Hiroyuki, Otaka, Asami, Hoshino, Atsushi, Iida, Shigeru, Nitasaka, Eiji, Watanabe, Nobuyoshi, Kuboyama, Tsutomu
Jazyk:	angličtina
Rok vydání:	2012
Předmět:	food and beverages EST genetic diversity DNA marker Note SSR morning glory
Zdroj:	Breeding Science
ISSN:	1347-3735 1344-7610
Popis:	Although Japanese morning glory (Ipomoea nil (L.) Roth.) has been used intensively for genetic studies, DNA markers have not been developed in Ipomoea nil sufficient to cover all chromosomes. Therefore, we conducted microsatellite (simple sequence repeats, SSR) marker development in I. nil for future genetic studies. From 92,662 expressed sequence tag (EST) sequences, 514 unique microsatellite-containing ESTs were identified. Primer pairs were designed automatically in 326 SSRs. Of 150 SSRs examined, 75 showed polymorphisms among strains. A phenogram based on the SSR genotypes revealed the genetic relation among seven Japanese morning glories from five different regions of the world and an ivyleaf morning glory (I. hederacea Jacq.). The developed SSR markers might be applicable for genetic studies of morning glories and their relatives.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::6394271f1b14c7e5803e35e30e745576 http://europepmc.org/articles/PMC3405949 Zobrazit plný text záznamu Plný text ve formátu PDF