Preparation and characterization of polyclonal antibodies against human chaperonin 10
| Autor: | M J, Somodevilla-Torres, N C, Hillyard, H, Morton, D, Alewood, J A, Halliday, P F, Alewood, D A, Vesey, M D, Walsh, A C, Cavanagh |
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| Rok vydání: | 2000 |
| Předmět: |
Immune Sera
Recombinant Fusion Proteins Blotting Western Carcinoma Molecular Sequence Data Short Communications Enzyme-Linked Immunosorbent Assay Precipitin Tests Antibodies Peptide Fragments Neoplasm Proteins Antibody Specificity Chaperonin 10 Animals Humans Immunization Amino Acid Sequence Rabbits Colorectal Neoplasms |
| Zdroj: | Cell stresschaperones. 5(1) |
| ISSN: | 1355-8145 |
| Popis: | Early pregnancy factor (EPF) has been identified as an extracellular homologue of chaperonin 10 (Cpn10), a heat shock protein that functions within the cell as a molecular chaperone. Here, we report the production of polyclonal antibodies directed against several different regions of the human Cpn10 molecule and their application to specific protein quantitation and localization techniques. These antibodies will be valuable tools in further studies to elucidate the mechanisms underlying the differential spatial and temporal localization of EPF and Cpn10 and in studies to elucidate structure and function. |
| Databáze: | OpenAIRE |
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