Tissue and T cell distribution of precursor and mature IL-16
| Autor: | G L, Chupp, E A, Wright, D, Wu, M, Vallen-Mashikian, W W, Cruikshank, D M, Center, H, Kornfeld, J S, Berman |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Interleukin-16 Adolescent CD3 Complex Molecular Sequence Data Antibodies Monoclonal CD8-Positive T-Lymphocytes Lymphocyte Activation Organ Specificity T-Lymphocyte Subsets Leukocytes Mononuclear Humans Amino Acid Sequence Protein Precursors Protein Processing Post-Translational |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 161(6) |
| ISSN: | 0022-1767 |
| Popis: | IL-16 is a novel cytokine, which is chemoattractant for CD4+ T cells, macrophages, and eosinophils. Recently, it was reported that IL-16 is synthesized as an approximately 80-kDa precursor molecule, pro-IL-16. Since little is known about the processing and tissue distribution of IL-16 and pro-IL-16, we investigated the distribution of IL-16 mRNA and protein in human lymphoid tissue. Northern blotting identified IL-16 mRNA predominantly in normal lymphoid organs, including PBMC, spleen, and thymus. Immunohistochemistry of human lymph node localized IL-16 protein to lymphocyte cytoplasm within T cell zones and occasionally in lymphocytes in B cell zones. Flow cytometric detection of intracellular IL-16 showed that70% of CD4+ and CD8+ T cells constitutively expressed IL-16 protein. Western blot analysis of PBMC revealed nearly all of this protein to be approximately 80-kDa pro-IL-16 in unstimulated PBMC, and upon cell activation, the amino terminus of pro-IL-16 is processed into multiple fragments. These results show that pro-IL-16 is widely and constitutively expressed and suggest that the amino terminus of the protein can be processed upon cell activation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|