| Autor: |
Hong-lin, Dong, Jin-liang, Xing, Jia-ze, An, Xiang-min, Yang, Xi-ying, Yao, Ke-feng, Dou, Zhi-nan, Chen |
| Rok vydání: |
2005 |
| Předmět: |
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| Zdroj: |
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 21(3) |
| ISSN: |
1007-8738 |
| Popis: |
To express secretively chimeric Fab antibody HAb18 (cFab) against human hepatocellular carcinoma in Pichia pastoris.Genes encoding CL chain and Fd fragment of cFab antibody HAb18 were subcloned into vectors pPIC9K and pPICZalphaA, respectively. After confirmed by DNA sequence analysis, the recombinant plasmids pPIC9K/CL and pPICZalphaA/Fd were transformed into the genome of Pichia pastoris GS115. Mut(+) multiple insert transformants were screened by G418 and Zeocin and then induced with 5 mL/L methanol to express cFab.4 days after methanol induction, 26 mg/L of the cFab fragment was detected in the culture supernatant. Western blot proved that the expressed protein could specifically bind with HAb18GEF antigen.The successful expression of cFab/HAb18 in Pichia pastoris lays the foundation for large-scale production and further application of the antibody. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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