| Autor: |
Hayden P, Huggins, Jacob S, Subash, Hamilton, Stoffel, Melissa A, Henderson, Jenna L, Hoffman, David S, Buckner, Madhu S, Sengupta, Peter R, Boag, Myon-Hee, Lee, Brett D, Keiper |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
J Cell Sci |
| ISSN: |
1477-9137 |
| Popis: |
Germ cells use both positive and negative mRNA translational control to regulate gene expression that drives their differentiation into gametes. mRNA translational control is mediated by RNA-binding proteins, miRNAs and translation initiation factors. We have uncovered the discrete roles of two translation initiation factor eIF4E isoforms (IFE-1, IFE-3) that bind 7-methylguanosine (m7G) mRNA caps during Caenorhabditis elegans germline development. IFE-3 plays important roles in germline sex determination (GSD), where it promotes oocyte cell fate and is dispensable for spermatogenesis. IFE-3 is expressed throughout the germline and localizes to germ granules, but is distinct from IFE-1 and PGL-1, and facilitates oocyte growth and viability. This contrasts with the robust expression in spermatocytes of IFE-1, the isoform that resides within P granules in spermatocytes and oocytes, and promotes late spermatogenesis. Each eIF4E is localized by its cognate eIF4E-binding protein (IFE-1:PGL-1 and IFE-3:IFET-1). IFE-3 and IFET-1 regulate translation of several GSD mRNAs, but not those under control of IFE-1. Distinct mutant phenotypes, in vivo localization and differential mRNA translation suggest independent dormant and active periods for each eIF4E isoform in the germline. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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