| Autor: |
Liton Kumar, Saha, Sujin, Kim, Habyeong, Kang, Salma, Akter, Kyungho, Choi, Tetsushi, Sakuma, Takashi, Yamamoto, Hiroyuki, Sasanuma, Kouji, Hirota, Jun, Nakamura, Masamitsu, Honma, Shunichi, Takeda, S, Dertinger |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Environmental and molecular mutagenesis. 59(6) |
| ISSN: |
1098-2280 |
| Popis: |
The micronucleus (MN) test has become an attractive tool both for evaluating the genotoxicity of test chemicals because of its ability to detect clastogenic and aneugenic events and for its convenience. As the MN assay has been mostly performed using only DNA repair-proficient mammalian cells, we believed that the comparison of the MN frequency between DNA repair-proficient and -deficient human cells may be an excellent indicator for detecting the genotoxic potential of test chemicals and for understanding their mode of action. To address this issue, the following five genes encoding DNA-damage-response (DDR) factors were disrupted in the TK6 B cell line, a human cell line widely used for the MN test: FANCD2, DNA polymerase ζ (REV3), XRCC1, RAD54, and/or LIG4. Using these isogenic TK6 cell lines, the MN test was conducted for four widely-used DNA-damaging agents: methyl methanesulfonate (MMS), hydrogen peroxide (H |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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