| Autor: |
J R, Barr, V L, Maggio, D G, Patterson, G R, Cooper, L O, Henderson, W E, Turner, S J, Smith, W H, Hannon, L L, Needham, E J, Sampson |
| Rok vydání: |
1996 |
| Předmět: |
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| Zdroj: |
Clinical chemistry. 42(10) |
| ISSN: |
0009-9147 |
| Popis: |
An enzymatic hydrolysis isotope dilution-mass spectrometric method was developed for reference quantification of specific proteins. The analytical procedure involved measuring a reproducibly hydrolyzed peptide (serving as the primary standard) unique to a specific protein. This new mass spectrometric method was evaluated by assessing the concentration of apolipoprotein (apo) A-I in the European Community Bureau of Reference (BCR) lyophilized Certified Reference Material (CRM 393). We used the method to make 96 measurements (4 replicate analyses of 4 enzymatic digests of 6 vials of BCR-CRM 393), which gave an average total protein mass of 1.048 mg (+/- 1.0% at 99% confidence limits). The total overall analytical CV was 3.95%. The results of this evaluation of our model approach to determine the concentration of a specific protein in a purified preparation demonstrated that our new mass spectrometric method can be used to measure apolipoproteins and other specific proteins without the use of epitopic immunoassay methods. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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