| Autor: |
G J, Wedemayer, I, Medintz, W W, Wong, J R, Scherer, R A, Mathies |
| Rok vydání: |
2001 |
| Předmět: |
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| Zdroj: |
BioTechniques. 30(1) |
| ISSN: |
0736-6205 |
| Popis: |
The collection and conversion of 4-color fluorescent genotyping data from capillary array electrophoresis microchip devices and its conversion to a format easily and rapidly analyzed by Genetic Profiler genotyping software is presented. Microchip fluorescence intensity data are acquired and stored as 4-color tab-delimited text. These files are converted to electrophoretic signal data (ESD) files using a utility program (TEXT-to-ESD) written in C. TEXT-to-ESD generates an ESD file by converting text data to binary data and then appending a 632-byte ESD-file trailer. Up to 96 ESD files are then assembled into a run folder and imported into Genetic Profiler, where data are reduced to 4-color electropherograms and analyzed. In this manner, DNA fragment sizing data acquired with our high-speed electrophoretic microchip devices can be rapidly analyzed using robust commercial software. Additionally, the conversion program allows sizing of data with Genetic Profiler that have been preprocessed using other third-party software, such as BaseFinder. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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