PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination
| Autor: | Thomas L, Clarke, Maria Pilar, Sanchez-Bailon, Kelly, Chiang, John J, Reynolds, Joaquin, Herrero-Ruiz, Tiago M, Bandeiras, Pedro M, Matias, Sarah L, Maslen, J Mark, Skehel, Grant S, Stewart, Clare C, Davies |
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| Rok vydání: | 2016 |
| Předmět: |
Protein-Arginine N-Methyltransferases
Time Factors RUVBL1 DNA repair Mice Transgenic homologous recombination Ataxia Telangiectasia Mutated Proteins Arginine Transfection Methylation 52BP1 Genomic Instability Lysine Acetyltransferase 5 Article Histones Mice Animals Humans DNA Breaks Double-Stranded Histone Acetyltransferases DNA Helicases Recombinational DNA Repair Acetylation arginine methylation HEK293 Cells ATPases Associated with Diverse Cellular Activities PRMT5 RNA Interference Carrier Proteins Tumor Suppressor p53-Binding Protein 1 TIP60 Protein Processing Post-Translational HeLa Cells |
| Zdroj: | Molecular Cell |
| ISSN: | 1097-4164 |
| Popis: | Summary Protein post-translation modification plays an important role in regulating DNA repair; however, the role of arginine methylation in this process is poorly understood. Here we identify the arginine methyltransferase PRMT5 as a key regulator of homologous recombination (HR)-mediated double-strand break (DSB) repair, which is mediated through its ability to methylate RUVBL1, a cofactor of the TIP60 complex. We show that PRMT5 targets RUVBL1 for methylation at position R205, which facilitates TIP60-dependent mobilization of 53BP1 from DNA breaks, promoting HR. Mechanistically, we demonstrate that PRMT5-directed methylation of RUVBL1 is critically required for the acetyltransferase activity of TIP60, promoting histone H4K16 acetylation, which facilities 53BP1 displacement from DSBs. Interestingly, RUVBL1 methylation did not affect the ability of TIP60 to facilitate ATM activation. Taken together, our findings reveal the importance of PRMT5-mediated arginine methylation during DSB repair pathway choice through its ability to regulate acetylation-dependent control of 53BP1 localization. Graphical Abstract Highlights • PRMT5 is a regulator of homologous recombination-mediated double-strand break repair • PRMT5 methylates RUVBL1 at R205, regulating TIP60-mediated histone acetylation • Loss of RUVBL1 methylation leads to 53BP1 retention at break ends • Arginine methylation crosstalks with histone acetylation to regulate repair pathway choice Clarke et al. show that methylation of RUVBL1 by the arginine methyltransferase PRMT5 is required for homologous recombination-mediated double-strand break repair by promoting TIP60-mediated histone H4K16 acetylation. Loss of PRMT5 activity and defective RUVBL1 methylation leads to 53BP1 retention, increased sensitivity to DNA damaging agents, and genome instability. |
| Databáze: | OpenAIRE |
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