| Autor: |
Jack W D, Blackburn, Darius H C, Lau, Elaine Y, Liu, Jessica, Ellins, Angela M, Vrieze, Emily N, Pawlak, Jimmy D, Dikeakos, Bryan, Heit |
| Rok vydání: |
2018 |
| Předmět: |
|
| Zdroj: |
European journal of immunology. 49(4) |
| ISSN: |
1521-4141 |
| Popis: |
Efferocytosis is essential for homeostasis and prevention of the inflammatory and autoimmune diseases resulting from apoptotic cell lysis. CD93 is a transmembrane glycoprotein previously implicated in efferocytosis, with mutations in CD93 predisposing patients to efferocytosis-associated diseases. CD93 is a cell surface protein, which is proteolytically shed under inflammatory conditions, but it is unknown how CD93 mediates efferocytosis or whether its efferocytic activity is mediated by the soluble or membrane-bound form. Herein, using cell lines and human monocytes and macrophages, we demonstrate that soluble CD93 (sCD93) potently opsonizes apoptotic cells but not a broad range of microorganisms, whereas membrane-bound CD93 has no phagocytic, efferocytic, or tethering activity. Using mass spectrometry, we identified α |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Plný text