[Obtaining diagnostic fluorescent monoclonal immunoglobulins against the V. cholerae 0139-serovar]

Autor: L P, Alekseeva, B L, Mazrukho, O V, Markina, O S, Chemisova, O I, Sal'nikova, E V, Ishina
Rok vydání: 2003
Předmět:
Zdroj: Klinicheskaia laboratornaia diagnostika. (12)
ISSN: 0869-2084
Popis: Below is given a procedure of the obtaining diagnostic fluorescent monoclonal immunoglobulin to detect cholera vibrios of O139 serovar. While obtaining preparations it was managed to determine optimal FTTS-MKA ratio, duration of their conjugation, series of fluorochrome. Test specimens of fluorescent monoclonal immunoglobulin provides intensive glow of V cholerae O139 cells in the working dilution 1:16-1:32. Tests of diagnostic FTTS-MKA on the great number of homologic and heterologic strains showed their strict specificity and high sensibility as to cholera vibrios of O139 serogroup.
Databáze: OpenAIRE