Measurement of underivatized propranolol enantiomers in serum using a cellulose-tris(3,5-dimethylphenylcarbamate) high-performance liquid chromatographic (HPLC) chiral stationary phase

Autor:	R J, Straka, R L, Lalonde, I W, Wainer
Rok vydání:	1988
Předmět:	Humans Reproducibility of Results Stereoisomerism Reference Standards Tromethamine Cellulose Propranolol Chromatography High Pressure Liquid
Zdroj:	Pharmaceutical research. 5(3)
ISSN:	0724-8741
Popis:	A commercially available high-performance liquid chromatographic (HPLC) chiral stationary phase (HPLC-CSP) has been used to measure serum levels of d- and l-propranolol. The HPLC-CSP is based upon cellulose-tris(3,5-dimethylcarbamate) and is able to stereochemically resolve d- and l-propranolol without precolumn derivatization using a mobile phase composed of hexane:2-propranol:N,N-dimethyloctylamine (92:8:0.01, v/v/v). Under these conditions the observed stereochemical resolution (alpha) of the two enantiomers was alpha = 2.2. A subject's concentration-time curve of the two isomers was determined following the ingestion of 160 mg racemic propranolol.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::602cf375025be03ddcec9e0584f362d5 https://pubmed.ncbi.nlm.nih.gov/3244632 Zobrazit plný text záznamu Full text from SpringerLink