[Isolation and identification of a Bacillus amyloliquefaciens YB-3 against Rhizoctonia solani]

Autor: Xiaofei, Zhu, Xiaoxia, Zhang, Yongchun, Niu, Yuansen, Hu, Yanchun, Yan, Haisheng, Wang
Rok vydání: 2011
Předmět:
Zdroj: Wei sheng wu xue bao = Acta microbiologica Sinica. 51(8)
ISSN: 0001-6209
Popis: An antagonistic bacterial strain YB-3 against Rhizoctonia solani was isolated from soils.Antagonistic strains were isolated by a reporter strain method. YB-3 was identified based on morphology observation, physiological and biochemical characterizations, Biolog, G + C content and 16S rDNA sequence analysis. The antagonistic spectrum and the properties of the inhibitor produced by Bacillus amyloliquefaciens YB-3 against plant pathogenic fungi and bacteria were investigated by means of plate two-way cultivation and disc diffusion method.The strain YB-3 against Rhizoctonia solani was identified as Bacillus amyloliquefaciens. The antagonistic results showed that it had distinctively inhibitive effects on 14 pathogenic fungi and 7 bacteria. In addition, it also had inhibitive effects on strains from genus Bacillus to which YB-3 belongs. Antagonistic properties of B. amyloliquefaciens YB-3 was thermostable, acid resistant, and protease sensitive.Bacillus amyloliquefaciens YB-3 was isolated and characterized which had distinctively inhibitive effects on Rhizoctonia solani and had broad-spectrum, highly efficient to plant pathogens.
Databáze: OpenAIRE