Proteomic analysis reveals that 14-3-3sigma is down-regulated in human breast cancer cells
| Autor: | A S, Vercoutter-Edouart, J, Lemoine, X, Le Bourhis, H, Louis, B, Boilly, V, Nurcombe, F, Révillion, J P, Peyrat, H, Hondermarck |
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| Rok vydání: | 2001 |
| Předmět: |
Exonucleases
Down-Regulation Proteins Breast Neoplasms Epithelial Cells Neoplasm Proteins Gene Expression Regulation Neoplastic 14-3-3 Proteins Protein Biosynthesis Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Exoribonucleases Biomarkers Tumor Tumor Cells Cultured Autoradiography Humans Protein Isoforms Electrophoresis Gel Two-Dimensional Breast |
| Zdroj: | Cancer research. 61(1) |
| ISSN: | 0008-5472 |
| Popis: | The class of molecular chaperones known as 14-3-3 is involved in the control of cellular growth by virtue of its apparent regulation of various signaling pathways, including the Raf/mitogen-activated protein kinase pathway. In breast cancer cells, the sigma form of 14-3-3 has been shown to interact with cyclin-dependent kinases and to control the rate of entry into mitosis. To test for a direct role for 14-3-3 in breast epithelial cell neoplasia, we have quantitated 14-3-3 protein levels using a proteomic approach based on two-dimensional electrophoresis and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF). We show here that 14-3-3sigma protein is strongly down-regulated in the prototypic breast cancer cell lines MCF-7 and MDA-MB-231 and in primary breast carcinomas as compared with normal breast epithelial cells. In contrast, levels of the alpha, beta, delta, or zeta isoforms of 14-3-3 were the same in both normal and transformed cells. The data support the idea that 14-3-3sigma is involved in the neoplastic transition of breast epithelial cells by virtue of its role as a tumor suppressor; as such, it may constitute a robust marker with clinical efficacy for this pathology. |
| Databáze: | OpenAIRE |
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