[DNA replication in HeLa cells after gamma irradiation. II. The periodicity of DNA replication in the process of giant cell formation]

Autor: T A, Sheĭkina, G B, Belostotskaia, A F, Lepëkhin
Rok vydání: 1978
Předmět:
Zdroj: Tsitologiia. 20(9)
ISSN: 0041-3771
Popis: HeLa G-63 cells irradiated by 5 krads of 6 degrees Co gamma-rays 1.5--2 hours after mitosis (G1-phase) were incubated in growth medium during 9 days. The number of proliferating and eliminated cells, the content of DNA per cell nucleus, and kinetics of the labeled cell fraction upon 3H-TdR continuous incubation were studied. It has been concluded that: during the giant cell growth the nuclear DNA is replicated periodically without following mitoses; 90% of cells are involved in such a cycle; the duration of the period of DNA synthesis in giant cells is the same as that in non-irradiated cells; the duration of the gap between two successive S-periods in giant cells is 2--6 times as long as the sum of G2+M+G1 periods in the normal non-irradiated cells.
Databáze: OpenAIRE