| Autor: |
S Iu, Ostrovskiĭ, I M, Artsukevich |
| Rok vydání: |
1989 |
| Předmět: |
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| Zdroj: |
Biokhimiia (Moscow, Russia). 54(11) |
| ISSN: |
0320-9725 |
| Popis: |
The pattern of kinetic behaviour of ethanolamine (EA), an ethanol structural analog, in the alcohol dehydrogenase reaction has been studied. EA has been shown to manifest a mixed type inhibition versus ethanol and a noncompetitive behaviour towards the second substrate, NAD. A graphical analysis of the experimental results as well as the construction of secondary graphs provide evidence in favour of a mechanism, according to which the interaction between EA and the enzyme results in a dead-end complex formation (ESI). A direct conversion into reaction products can be achieved only after EA separation from the complex. The Ki value for the E-EA complex is 1.3 mM; that for EA release from the E-EA is 1.8 mM. An analysis of competitive interactions with NAD showed these constants to be equal in values (2 mM). Taking account of real concentrations of tissue EA and of experimental values of Ki, a conclusion is drawn on possible participation of EA in the alcohol dehydrogenase reaction control. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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