| Autor: |
S C, Vashishtha, E M, Hawes, G, McKay, D J, McCann |
| Rok vydání: |
2000 |
| Předmět: |
|
| Zdroj: |
Drug metabolism and disposition: the biological fate of chemicals. 28(9) |
| ISSN: |
0090-9556 |
| Popis: |
1-Phenylimidazole was investigated as a potential model substrate with respect to formation of a quaternary ammonium-linked glucuronide (N(+)-glucuronide) at an aromatic type tertiary amine. A reference sample of the potential N(+)-glucuronide metabolite of 1-phenylimidazole was obtained by organic synthesis. The structural identity of the metabolite formed by incubation of 1-phenylimidazole with human liver microsomes was proven to be the N(+)-glucuronide by exhibiting the same HPLC retention time and electrospray ionization mass spectrum as the reference sample. The screening of 1-phenylimidazole against a panel of nine expressed human UDP-glucuronosyltransferases indicated the involvement of UGT1A3 and UGT1A4 in the formation of the N(+)-glucuronide metabolite. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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