Extraction of human nuclear DNA from feces samples using the QIAamp DNA Stool Mini Kit

Autor:	Nicholas, Vandenberg, Roland A H, van Oorschot
Rok vydání:	2002
Předmět:	Adult Feces Humans DNA Environment Forensic Medicine DNA Fingerprinting Polymerase Chain Reaction
Zdroj:	Journal of forensic sciences. 47(5)
ISSN:	0022-1198
Popis:	The use of a QIAamp DNA Stool Mini Kit (QIAGEN) for extracting human nuclear DNA from feces samples is reported. This method employs a stool lysis buffer and a unique matrix (InhibitEX tablet) to remove PCR inhibitory substances specific to feces samples. DNA extracted from various amounts of stool and from stool samples exposed to different environmental impacts was successfully amplified and typed using the Profiler Plus Amplification Kit and ABI PRISM 310 Genetic Analyser.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::5ed40affa28f5e4d50f088e7c2acfbe0 https://pubmed.ncbi.nlm.nih.gov/12353586 Zobrazit plný text záznamu