Characterization of a receptor for human monocyte-derived neutrophil chemotactic factor/interleukin-8
| Autor: | P M, Grob, E, David, T C, Warren, R P, DeLeon, P R, Farina, C A, Homon |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Chemotactic Factors
Neutrophils Wheat Germ Agglutinins Interleukins Cell Membrane Interleukin-8 Temperature Affinity Labels Cell Differentiation Binding Competitive Monocytes Recombinant Proteins Cell Line Receptors Interleukin-8A Iodine Radioisotopes Molecular Weight Chemotaxis Leukocyte Kinetics Mice Lectins Animals Humans Receptors Immunologic Glycoproteins |
| Zdroj: | The Journal of biological chemistry. 265(14) |
| ISSN: | 0021-9258 |
| Popis: | Monocyte-derived neutrophil chemotactic factor/interleukin-8 (MDNCF/IL-8) is an 8,000-dalton protein produced by monocytes which exhibits activity as a chemoattractant for neutrophils with maximal activity achieved at a concentration of 50 ng/ml. This polypeptide has been iodinated by chloramine-T methodology (350 Ci/mM), and specific receptors for MDNCF/IL-8 have been detected on human neutrophils, U937 cells, THP-1 cells, and dimethyl sulfoxide-differentiated HL-60 cells. The binding of MDNCF/IL-8 to human neutrophils is not inhibited by interleukin-1 alpha, tumor necrosis factor-alpha, insulin, or epidermal growth factor. In addition, chemoattractants such as C5a, fMet-Leu-Phe, leukotriene B4, and platelet-activating factor fail to inhibit binding, suggesting that MDNCF/IL-8 utilizes a unique receptor. The receptor for MDNCF/IL-8 is apparently glycosylated since ligand binding is inhibited by the presence of wheat germ agglutinin, a lectin with a binding specificity for N-acetylglucosamine and neuraminic acid. Steady state binding experiments indicate Kd values of 4 and 0.5 nM and receptor numbers of 75,000 and 7,400 for human neutrophils and differentiated HL-60 cells, respectively. 125I-MDNCF/IL-8 bound to human neutrophils is rapidly internalized and subsequently released from cells as trichloroacetic acid-soluble radioactivity. Affinity labeling experiments suggest that the human neutrophil MDNCF/IL-8 receptor exhibits a mass of approximately 58,000 daltons. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|