| Autor: |
Alexandr N, Zinchuk, Olga A, Golubovska, Borys A, Herasun, Andrii M, Zadorozhnyi, Oleksandr B, Herasun |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Wiadomosci lekarskie (Warsaw, Poland : 1960). 73(10) |
| ISSN: |
0043-5147 |
| Popis: |
The aim: Was a trial of intradermal immunization with native autoleukocytes as a curative vaccine.Materials and methods: Thus, 3-shot series vaccination by means of intradermal injection of autoleukocytes with 30 - 40 days interval was conducted for patients who, in spite of continuous (at least 2 years) therapy with nucleotide analogue, experienced HBV DNA reproduction. For this procedure, 80 - 100 ml of a patient's heparinized venous blood was being precipitated at the temperature 37оС for 120 - 140 minutes, after blood plasma was being centrifuged at 450g for 8 minutes. The precipitate was resuspended in 1 - 1.5 ml of blood serum and injected intradermally in the region of the back.Results: Viral load decreased in all patients even after single immunization; it was possible to achieve a negative result by ultrasensitive PCR method in 23.33% of patients in the group of patients who did not respond adequately to antiviral therapy.Conclusions: In patients with chronic hepatitis B, intradermal immunization with autoleukocytes has a significant impact on intensity of virus replication. It is confirmed by a considerable reduction of DNA HBV amount in patients, in whom antiviral therapy was stopped before immunization. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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