[An experimental luminescent immunochromatographic system]

Autor: S P, Iarkov, S N, Skopinskaia, I V, Shilenko, V N, Zlobin
Rok vydání: 2009
Předmět:
Zdroj: Vestnik Rossiiskoi akademii meditsinskikh nauk. (3)
ISSN: 0869-6047
Popis: Luminescent immunochromatographic assay for antigens of microorganisms was developed to detect antigens and cells of microorganisms, toxins. Luminescent submicrone latex particles, quantum labels, ganglioside-containing liposomes with sulforhodamine-01 in the internal volume were used as a luminescent mobile phase for conjugation with antibodies. Devices for registration of luminescent immunochromatograms were developed. Submicrone latexes were shown to have advantages over quantum labels, ganglioside-containing liposomes with sulforhodamine-101 and colloid gold for immunochromatographic procedures. Sensitivity of luminescent latex-based immunochromatographic indicator element was 3-30 that elements based on colloid gold conjugates for identical microorganisms and elements.
Databáze: OpenAIRE