Clinical validation of the influence of P-glycoprotein on technetium-99m-sestamibi uptake in malignant tumors
| Autor: | L, Kostakoglu, N, Elahi, P, Kïratlï, S, Ruacan, I, Sayek, E, Baltalï, A, Sungur, M, Hayran, C F, Bekdik |
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| Rok vydání: | 1997 |
| Předmět: |
Adult
Male Technetium Tc 99m Sestamibi Lung Neoplasms Carcinoma Ductal Breast Breast Neoplasms Middle Aged Immunohistochemistry Drug Resistance Multiple Neoplasm Proteins Carcinoma Medullary Antineoplastic Combined Chemotherapy Protocols Carcinoma Squamous Cell Humans Female ATP Binding Cassette Transporter Subfamily B Member 1 Prospective Studies Carcinoma Small Cell Radiopharmaceuticals Radionuclide Imaging Aged |
| Zdroj: | Journal of nuclear medicine : official publication, Society of Nuclear Medicine. 38(7) |
| ISSN: | 0161-5505 |
| Popis: | We prospectively studied 48 patients with either breast cancer (30 patients) or lung cancer (18 patients) to ascertain the relationship between the degree of accumulation of 99mTc-sestamibi and the expression of p-glycoprotein in tumor tissues.During initial presentation (37 patients) or post-therapy evaluation (11 patients), the patients underwent contemporaneous 99mTc-sestamibi imaging and biopsy (30 patients) or surgery (18 patients). The interval between surgery/biopsy and imaging varied between 3 and 15 days. All patients had radiologically detectable tumors. Immunohistochemical studies were performed on paraffin sections using a monoclonal antibody, JSB-1, developed against the internal epitope of p-glycoprotein. Tumor-to-background ratios were correlated with the level of p-glycoprotein expression determined by immunohistochemical studies.Our results showed an inverse correlation between the tumor-to-background ratios of 99mTc-sestamibi and the density of p-glycoprotein expression in tumor tissues. The values for the tumor-to-background ratios were significantly lower for those tumors expressing p-glycoprotein at high levels than those with scattered and no expression (p0.01 and p0.001, respectively).Although our results warrant further studies at the molecular level using PCR techniques after the extraction of mRNA, our data strongly suggest that 99mTc-sestamibi imaging is useful to noninvasively determine the presence of multidrug resistance in patients with malignant tumors. |
| Databáze: | OpenAIRE |
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