| Autor: |
Chen, Zhang, Yang-Wenyi, Liu, Min, Chen, Shiyao, Min, Jiabao, Mao, Qin, Li, Zhangcai, Chi |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Cell biology internationalREFERENCES. 46(9) |
| ISSN: |
1095-8355 |
| Popis: |
Tympanosclerosis (TS) is a result of long-standing middle ear inflammation characterized by fibroblasts ossification. Fibrosis is the last revertible stage in the progress of middle ear inflammation to TS. It was hypothesized that chronic hypoxia could be modulating fibrosis, which in turn additionally further aggravated hypoxia via decreasing oxygen diffusion. However, the effects of hypoxia on osteoinductive activity of fibroblasts have not been explored. Herein, we purposed to explore the role of hypoxia in osteogenic differentiation of fibroblasts derived from TS. The expression of bone morphogenetic protein-2 (BMP-2), hypoxia-inducible factor-1α (HIF-1α), and Vimentin in the human surgical specimens of tympansclerosis was investigated by immunofluorescent staining. Furthermore, cultured fibroblasts were stratified into the following study groups: control, 25, 50, and 100 μM cobaltous chloride (CoCl |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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