Inclusion body myositis: investigation of the mumps virus hypothesis by polymerase chain reaction

Autor:	S A, Fox, B K, Ward, P D, Robbins, F L, Mastaglia, N R, Swanson
Rok vydání:	1996
Předmět:	Adult Male Base Sequence Myositis Muscles Molecular Sequence Data Middle Aged Models Biological Polymerase Chain Reaction Sensitivity and Specificity Myositis Inclusion Body Mumps virus Humans RNA Viral Female Oligonucleotide Probes Aged
Zdroj:	Musclenerve. 19(1)
ISSN:	0148-639X
Popis:	Inclusion body myositis (IBM) is a distinctive form of chronic inflammatory myopathy characterized pathologically by the finding of rimmed vacuoles and 15-18nm microtubular filamentous inclusions in muscle fiber nuclei and cytoplasm. The observation that these filaments resembled nucleocapsids of the paramyxovirus group and showed immunoreactivity with mumps virus (MV) antibodies has led to a long-standing postulate that IBM may be a "slow" mumps infection. We searched for the presence of MV RNA in 34 muscle biopsies (17 frozen and 17 paraffin-embedded) from 18 patients with IBM and 43 control biopsies (mainly from patients with other forms of inflammatory myopathy) using a polymerase chain reaction (PCR). The MV PCR was shown to be sensitive and specific for MV strains (including J-L) and the integrity of muscle RNA extracts was confirmed by PCR detection of constitutive Ableson tyrosine kinase mRNA. MV RNA was not found in any biopsy from the IBM group nor any of the control cases. Our results therefore do not support the mumps hypothesis for IBM.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::5d9cbab4a281f4fc2ee133650b5a06a8 https://pubmed.ncbi.nlm.nih.gov/8538666 Zobrazit plný text záznamu Plný text