Popis: |
The activities of indicative enzymes involved in the dihydroxyacetone, glycolytic and pentose phosphate pathways, the citric acid cycle and the glyoxylate shunt were studied comparatively in the yeast Candida methylica growing in media with methanol, glucose and ethanol. Differences in the enzyme levels indicate that the pathways of substrate metabolism in methylotrophic yeast cells are regulated in an alternative and complicated manner. The nature of a carbon source, its concentration in the medium and the general direction of metabolic processes which, in turn, depend on the growth phase of the methylotrophic yeast are important regulatory factors of enzyme synthesis and activity. |