| Autor: |
Zhengyuan, Zhou, Rebecca, Meshaw, Michael R, Zalutsky, Ganesan, Vaidyanathan |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
J Nucl Med |
| ISSN: |
1535-5667 |
| Popis: |
Single-domain antibody fragments (sdAbs) are promising vectors for immuno-PET; however, better methods for labeling sdAbs with (18)F are needed. Herein, we evaluate a site-specific strategy using an (18)F residualizing motif and the anti–epidermal growth factor receptor 2 (HER2) sdAb 5F7 bearing an engineered C-terminal GGC tail (5F7GGC). Methods: 5F7GGC was site-specifically attached with a tetrazine-bearing agent via thiol-maleimide reaction. The resultant conjugate was labeled with (18)F by inverse electron demand Diels–Alder cycloaddition with a trans-cyclooctene attached to 6-(18)F-fluoronicotinoyl moiety via a renal brush border enzyme-cleavable linker and a PEG(4) chain ((18)F-5F7GGC). For comparisons, 5F7 sdAb was labeled using the prototypical residualizing agent, N-succinimidyl 3-(guanidinomethyl)-5-(125)I-iodobenzoate (iso-(125)I-SGMIB). The 2 labeled sdAbs were compared in paired-label studies performed in the HER2-expressing BT474M1 breast carcinoma cell line and athymic mice bearing BT474M1 subcutaneous xenografts. Small-animal PET/CT imaging after administration of (18)F-5F7GGC in the above mouse model was also performed. Results: (18)F-5F7GGC was synthesized in an overall radiochemical yield of 8.9% ± 3.2% with retention of HER2 binding affinity and immunoreactivity. The total cell-associated and intracellular activity for (18)F-5F7GGC was similar to that for coincubated iso-(125)I-SGMIB-5F7. Likewise, the uptake of (18)F-5F7GGC in BT474M1 xenografts in mice was similar to that for iso-(125)I-SGMIB-5F7; however, (18)F-5F7GGC exhibited significantly more rapid clearance from the kidney. Small-animal PET/CT imaging confirmed high uptake and retention in the tumor with very little background activity at 3 h except in the bladder. Conclusion: This site-specific and residualizing (18)F-labeling strategy could facilitate clinical translation of 5F7 anti-HER2 sdAb as well as other sdAbs for immuno-PET. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
