| Autor: |
V V, Kulikov, O B, Drize, E B, Nikashina, M A, Shliankevich, V S, Shapot |
| Rok vydání: |
1984 |
| Předmět: |
|
| Zdroj: |
Biokhimiia (Moscow, Russia). 49(5) |
| ISSN: |
0320-9725 |
| Popis: |
The role of virus SV40 T-antigen in the induction of cell DNA synthesis during its incorporation into cell liposomes was studied, using monolamellar liposomes obtained by phase reversal with incorporated highly purified T-antigen. Immunofluorescence studies revealed that T-antigen effectively penetrates inside the cells and after 10 hours is accumulated in the nuclei, where its level remains unchanged for 24 hours. Injections of purified T-antigen into the renal cells of serum-starved CV1 monkeys resulted in an almost 10-fold increase in the number of DNA-synthesizing cells 18 hours after the exposure. The same effect was observed during stimulation of a 10% serum culture. Removal of T-antigen from the preparation by specific immunoadsorption eliminated this effect. Centrifugation of cells grown in the presence of bromodeoxyuridine in a CsCl gradient was used to demonstrate the replicative type of cell DNA synthesis during T-antigen induction. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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