Optimization of ultrasonic-assisted extraction of polyphenols from the polyherbal formulation of
| Autor: | Rosa Martha, Pérez Gutiérrez, Felipe Fernando, Martínez Jerónimo, José Guadalupe, Contreras Soto, Alethia, Muñiz Ramírez, María Fernanda, Estrella Mendoza |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Heliyon |
| ISSN: | 2405-8440 |
| Popis: | The Cinnamomum verum (CV), Origanum majorana (CM), and Origanum vulgare (OV) have been used in traditional medicine in several regions of México for their anti-diabetic properties. In this study investigated the variables of ultrasound-assisted extraction for the polyphenolic compounds from the combination of these plants and explore their potential antidiabetic activities on glucose-induced-diabetic zebrafish. Determined the optimum conditions for ultrasonic-assisted extraction (UAE) to maximum recovery amounts of phenolic compounds from the extract of these plants. Polyphenols were detected in the extracts using HPLC-DAD-analysis. Extracts were evaluated on zebrafish exposed to high glucose concentration (110 mM) for two weeks. Results showed second-order polynomial mathematical models with a high coefficient of determination (R2 > 0.9564). Optimized extraction conditions for UAE from the combination of the 3 plants (COV) were as follows: 66.03%, ethanol, 28.87 min, and 21.51 mL/g for maximal flavonoids extraction. Used the same optimal extraction conditions for CV, CM, and OV. Results from LC-MS/MS indicated 9 polyphenolic compounds in CV, 12 in CM, and 6 in OV, the content of total polyphenols was 310.28, 90.42, and 126.74 mg GAE 100 g−1 dry weight, respectively. However, hyperglycemic fish showed an increase in cholesterol and triglyceride levels whereas extracts completely prevented these metabolic alterations. COV showed higher anti-diabetic ability than CV, CM, and OV, suggesting a synergistic effect between them. Our investigation developed a new herbal formulation of Cinnamomum verum; Origanum majorana; Origanum vulgare that has proven effective in animals with type 2 diabetes will form a new class of supplements to treat diabetic complications. Polyherbal formulation; Ultrasound-assisted extraction; Response surface technology; Cinnamomum verum; Origanum majorana; Origanum vulgare; T2DM zebrafish model. |
| Databáze: | OpenAIRE |
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