| Autor: |
A I, Glukhov, S A, Gordeev, M L, Al'tshuler, I E, Zykova, S E, Severin |
| Rok vydání: |
2003 |
| Předmět: |
|
| Zdroj: |
Klinicheskaia laboratornaia diagnostika. (7) |
| ISSN: |
0869-2084 |
| Popis: |
Causative agents of plague, i.e. bacterium Yersina pestis (in the subcutaneous tissues of rodents) and their cutaneous parasites need to be isolated to enable plague prevention. A comparatively new method of polymerase chain reaction (PCR) opens up new possibilities of determining Y. pestis just within several hours and without any cultivation. The article contains a description of the PCR-method, which makes it possible to distinguish the culture of Y. pestis from cultures of other microorganism, including speci of Yersina. The method is of the cluster-type, i.e. it is made up of subsequent PC reactions with the substrate for the second reaction being the product of the first one. The cluster nature of the method preconditions a higher sensitivity and specificity versus the ordinary PCR. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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