Phenotypic and genotypic characterization of competitive exclusion products for use in poultry

Autor:	R D, Wagner, D D, Paine, C E, Cerniglia
Rok vydání:	2003
Předmět:	Bacteria Anaerobic Databases Genetic Food Microbiology Animals Chickens Ribotyping Sensitivity and Specificity Poultry Diseases Bacterial Typing Techniques
Zdroj:	Journal of applied microbiology. 94(6)
ISSN:	1364-5072
Popis:	Phenotypic and genotypic bacteria identification methods were compared for their efficacy in determining the composition of competitive exclusion (CE) products.Phenotypic methods used for bacterial identification were fatty acid methyl ester profiles, biochemical assays and carbohydrate utilization profiles. Genotypic methods were MicroSeq16S rRNA sequence analysis and BLAST searches of the GenBank sequence database. Agreement between phenotypic and genotypic methods for identification of bacteria isolated from the Preempt CE product was 20%. A defined test mixture of bacteria was identified to the species level 100% by BLAST analysis, 64% by MicroSeq and 36% by phenotypic techniques.The wide range of facultative and obligate anaerobic bacteria present in a CE product are more accurately identified with 16S rRNA sequence analyses than with phenotypic identification techniques.These results will provide guidelines for manufacturers of CE products to submit more reliable product information for market approval by regulatory agencies.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::5ccdb0c645bb657738003186f7ca767c https://pubmed.ncbi.nlm.nih.gov/12752820 Zobrazit plný text záznamu Plný text