| Autor: |
R J, Planta, A J, Brown, J L, Cadahia, M E, Cerdan, M, de Jonge, M E, Gent, A, Hayes, C P, Kolen, L J, Lombardia, M, Sefton, S G, Oliver, J, Thevelein, H, Tournu, Y J, van Delft, D J, Verbart, J, Winderickx |
| Rok vydání: |
1999 |
| Předmět: |
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| Zdroj: |
Yeast (Chichester, England). 15(4) |
| ISSN: |
0749-503X |
| Popis: |
The European Functional Analysis Network (EUROFAN) is systematically analysing the function of novel Saccharomyces cerevisiae genes revealed by genome sequencing. As part of this effort our consortium has performed a detailed transcript analysis for 250 novel ORFs on chromosome XIV. All transcripts were quantified by Northern analysis under three quasi-steady-state conditions (exponential growth on rich fermentative, rich non-fermentative, and minimal fermentative media) and eight transient conditions (glucose derepression, glucose upshift, stationary phase, nitrogen starvation, osmo-stress, heat-shock, and two control conditions). Transcripts were detected for 82% of the 250 ORFs, and only one ORF did not yield a transcript of the expected length (YNL285w). Transcripts ranged from low (62%), moderate (16%) to high abundance (2%) relative to the ACT1 mRNA. The levels of 73% of the 206 chromosome XIV transcripts detected fluctuated in response to the transient states tested. However, only a small number responded strongly to the transients: eight ORFs were induced upon glucose upshift; five were repressed by glucose; six were induced in response to nitrogen starvation; three were induced in stationary phase; five were induced by osmo-stress; four were induced by heat-shock. These data provide useful clues about the general function of these ORFs and add to our understanding of gene regulation on a genome-wide basis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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