| Autor: |
Risa, Zulfiana, Suharjono, Kuntaman |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Journal of basic and clinical physiology and pharmacologyReferences. 32(4) |
| ISSN: |
2191-0286 |
| Popis: |
In this study, isolates collected in the Microbiology Laboratory of Dr. Seotomo Surabaya Hospital during May-September 2019. Then, the dilution method was carried out to determine the minimum inhibition concentration for resistant-rifampicin and dilution to determine the inhibition zone diameter. After that, DNA extraction was carried out from rifampicin-susceptible isolates as a control and resistant-rifampicin isolates followed by identification ofThere were nine isolates studied. They were four resistant-rifampicin isolates and four susceptible-rifampicin isolates. In four rifampicin-resistant isolates, the most frequent mutations that occurred was His-481 codon (75%) followed by the Ile-527 codon (25%). Rifampicin-susceptible isolates mutated in Pro-475 and Asn-474 codons. One rifampicin-resistant isolate had two mutations in codons Ile-527 and Asn-474.The type of mutation that causes the most rifampicin resistance was a missense mutation. The susceptible-rifampicin isolate experienced silent mutations. There was a relation between the type of missense mutation of |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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