| Autor: |
Chenyang, Ji, Fuli, Min, Weiqiang, Liang, Yuhong, Chen, Shujuan, Pan, Lianjie, Bin, Shihong, Chen, Zhe, Wei, Tao, He, Yanhui, Gu, Jinming, Zhang |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
BJU international. 107(10) |
| ISSN: |
1464-410X |
| Popis: |
• To investigate the feasibility of tissue-engineered corpus cavernosum (TECC) with muscle-derived stem cells (MDSCs) as seed cells and determine the growth potential in vivo.• Acellular corporal collagen matrices (ACCMs) were obtained from adult rabbit penis by a cell removal procedure. MDSCs were separated and purified using a digestion method and Preplate technique, then seeded on ACCMs at a concentration of 30 × 10(6) cells/mL to construct TECCs. After 5 days of culture, seeded ACCMs were implanted with albuginea of rabbits. The implants were retrieved at 2, 4 and 6 months after implantation. • Histochemistry, immunohistochemisry and scanning electron microscopy were performed to analyse the morphological characteristics of the TECCs.• The decellularization process successfully extracted all cellular components while preserving the original collagen fibres. • Histological analyses of the explants at all time points in the experimental group had more cells and better arranged growth than the control group. α-Smooth muscle actin and endothelial nitric oxide synthase-positive cells were more prevalent in the experimental group.• Our study showed that MDSCs can be seeded on three-dimensional ACCM scaffolds and develop tissues that are similar to native normal corpus cavernosum. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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