PtdIns 4-kinasebeta and neuronal calcium sensor-1 co-localize but may not directly associate in mammalian neurons

Autor:	S E, Bartlett, A J, Reynolds, M, Weible, A, Jeromin, J, Roder, I A, Hendry
Rok vydání:	2000
Předmět:	Mice Mice Inbred BALB C Saccharomyces cerevisiae Proteins Animals Newborn Ganglia Spinal Calcium-Binding Proteins Neuronal Calcium-Sensor Proteins Neuropeptides Neurites Animals Embryo Mammalian 1-Phosphatidylinositol 4-Kinase Cells Cultured
Zdroj:	Journal of neuroscience research. 62(2)
ISSN:	0360-4012
Popis:	It was recently demonstrated that the yeast homologue of phosphatidylinositol 4-kinasebeta PIK1 is directly associated with frq1, the yeast homologue of mammalian neuronal calcium sensor-1 (NCS-1) (Hendricks et al., [1999] Nat. Cell Biol. 1:234- 241). This was a novel finding and suggests that a calcium binding protein activates and regulates PtdIns 4-kinasebeta. This finding had not been shown in mammalian cells and both PtdIns 4-kinasebeta and NCS-1 have been shown to have important roles in the regulation of exocytotic release associated with neurotransmission. The aims of this study were to determine if PtdIns 4-kinasebeta and NCS-1 directly associate in mammalian neural tissues. We show that the immunostaining pattern for PtdIns 4-kinasebeta and NCS-1 is co-localized throughout the neurites of newborn cultured dorsal root ganglia (DRG) neurons but not in E13 DRG neurons. We then provide biochemical evidence that PtdIns 4-kinasebeta may not be in physical association with NCS-1 in mammalian nervous tissue unlike that previously reported in yeast.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::5c18b00db58720a545f6babd27948d4b https://pubmed.ncbi.nlm.nih.gov/11020214 Zobrazit plný text záznamu Plný text