HLA typing by next-generation sequencing - getting closer to reality

Autor:	C, Gabriel, D, Fürst, I, Faé, S, Wenda, C, Zollikofer, J, Mytilineos, G F, Fischer
Rok vydání:	2014
Předmět:	HLA Antigens Research Design Histocompatibility Testing High-Throughput Nucleotide Sequencing Humans Sequence Analysis DNA Algorithms Software
Zdroj:	Tissue antigens. 83(2)
ISSN:	1399-0039
Popis:	Next generation sequencing (NGS) denotes novel sequencing technologies that enable the generation of a large number of clonal sequences in a single sequencing run. NGS was initially introduced for whole genome sequencing and for quantitation of viral variants or genetic mutations in tumor tissues; more recently, the potential for high resolution HLA typing and high throughput analyses has been explored. It became clear that the complexity of the HLA system implicates new challenges, especially for bioinformatics. From an economical point of view, NGS is becoming increasingly attractive for HLA typing laboratories currently relying on Sanger based sequencing. Realizing the full potential of NGS will require the development of specifically adapted typing strategies and software algorithms. In the present review, three laboratories that were among the first to perform HLA-typing using different NGS platforms, the Roche 454, the Illumina Miseq and the Ion Torrent system, respectively, give an overview of these applications and point out advantages and limitations.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::5be5d53d8bc62d536eb6fa9156e3ad74 https://pubmed.ncbi.nlm.nih.gov/24447174 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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