Altered growth patterns in vitro of human papillary transitional carcinoma cells
| Autor: | Reznikoff, C. A., Gilchrist, K. W., Norback, D. H., Cummings, K. B., Ertürk, E., Bryan, G. T. |
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| Jazyk: | angličtina |
| Rok vydání: | 1983 |
| Předmět: |
Adult
Male Carcinoma Transitional Cell Adolescent Biopsy Infant Middle Aged urologic and male genital diseases female genital diseases and pregnancy complications Carcinoma Papillary Culture Media Urinary Bladder Neoplasms Child Preschool Humans Female Ureter Child Cell Division Cells Cultured Research Article Aged |
| Popis: | In vitro growth patterns and morphologic characteristics of five low-grade human papillary transitional cell carcinomas (TCCs) were compared and contrasted with those of normal human urothelial cells in culture. Biopsies of TCC were performed by transurethral resection. Specimens of normal human ureters were obtained surgically. Singly dispersed TCC cells grew in 0.3% agarose semisolid medium with a cloning efficiency ranging from 0.02% to 0.71%. Singly dispersed normal ureteral urothelial cells under the same conditions did not form colonies in 0.3% agarose. Neither singly dispersed TCC nor normal urothelial cells formed colonies when plated on collagen-gel substrates. In primary explant culture, normal human urothelial cells grew rapidly, to form tightly adherent flat sheets of apparently nonmotile cells. Autoradiographic labeling with 3H-thymidine of growing cultures of normal urothelial cells showed cell division primarily in the zones of growth near the explant. Outgrowth of TCC from primary explants was loosely adherent. One TCC explant culture gave rise to a continuous suspension culture. Numerous multilayered cellular formations of fronds, nodules, and "walls" were seen around the periphery of TCC explant colonies. Autoradiography showed that these multilayered areas of TCC growth contained actively dividing cells. The altered ability of papillary TCC to form superficial multilayered formations in vitro distinguishes them from normal human urothelium and reflects the morphologic characteristic of this tumor type in vivo. |
| Databáze: | OpenAIRE |
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