Assaying peptide translocation by the peptide transporter TAP

Autor:	Marlieke L M, Jongsma, Jacques, Neefjes
Rok vydání:	2013
Předmět:	Halogenation Chloramines Catalysis Permeability Cell Line Tosyl Compounds Protein Transport Bacterial Proteins Microsomes Streptolysins Humans ATP-Binding Cassette Transporters Peptides Fluorescent Dyes
Zdroj:	Methods in molecular biology (Clifton, N.J.). 960
ISSN:	1940-6029
Popis:	MHC class I molecules display peptides at the cell surface that are mostly derived from cytosolic or nuclear proteins. Since peptide loading of MHC class I molecules occurs in the ER lumen, cytosolic peptides have to pass the ER membrane. The peptide transporter TAP translocates peptides over this ER membrane which is critical for successful MHC class I antigen presentation. How peptide translocation by TAP can be assayed and inhibitors of chemical or viral origin can be identified, will be described here.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::5b74e4325d6f0f0431af73b460a03d73 https://pubmed.ncbi.nlm.nih.gov/23329478 Zobrazit plný text záznamu